Friday, May 15, 2020
The Free English Essay Samples Stories
<h1> The Free English Essay Samples Stories </h1> <p>There's various papers finished by them. There's no perfect arrangement about how to form a viable article. These days it is incredibly hard to find a reliable paper composing administration. A genuine model article will assist it with getting possible to examine exposition structure and blueprints notwithstanding composing and reference stylethere are sure things which make an enticing paper. </p> <p>The subject of your article is indispensable. Our papers are quite often genuine and totally liberated from any copyright infringement. On the off chance that it's despite everything genuine that you could barely compose awesome expositions, you can see absolutely free article papers on the web. As a result, we propose that you work on composing test articles on a few themes. </p> <p>Every understudy requests help with schoolwork now and again. In this way, numerous understudies and workers choose to obtain modest paper as opposed to composing it themselves. </p> <h2> The History of Free English Essay Samples Refuted</h2> <p>You see, the shows of English papers are to some degree more equation based than you may might suspect and, in an assortment of ways, it very well may be as simple as tallying to five. Indeed, even the most notable models need setting. Unique content non-scholarly style in these kinds of countries. There'll be decision given from various subjects. </p> <p>Introduction article sections are focal in any piece of composing for the explanation they give perusers advance insights concerning the substance and the expectation behind the material. You don't should be worried about your composing assignments any more. On the off chance that you find that the author didn't give unequivocally what you expected, demand an amendment, and we'll make the remedies. In addition, our English-talking journalists ensure each request has unique substance and a suitable structure. </p> <h2> The Unexpected Truth About Free English Essay Samples </h2> <p>Even however the end passage comes at the finish of your paper it should not be viewed as an untimely idea. The numerous segments of the amalgamation exposition must be extended in the best conceivable design and it's best clarified through blend paper models. With respect to end, there shouldn't be any new focuses in it. </p> & lt;p>Please recognize the conditions wherein all of these reserve types might be proper. Additionally, keeping your layout around all through the whole procedure will assist you with remaining sorted out and help you in verifying you remember anything. Giving blood to wrap up. A particular contentious exposition model on drugs in this day and age and their impact on the general public. </p> <h2> Free English Essay Samples - Dead or Alive? </h2> <p>Your passages don't interface each other's significance notwithstanding the entire idea of your article may be endless. It's feasible for you to peruse all our 730 composing worksheets in thumbnail see, and that implies you don't need to look out for any of them to load to procure a thought regarding what they're similar to. A significant thought to remember is to be sure you don't have a thing in the exposition which isn't identified with your point. All out image of intriguing and might not. </p> <p> ;Our prepared specialists will complete an intensive assessment of your home and existing pipes challenges, providing you with a total, full gauge on the work expected to repipe your home. You won't have to leave your home. On the off chance that you dwell in a house that has been fabricated over 25 decades past, there's a great likelihood that the water pipes in your home are produced using excited material. Rather than astounding estimation of high phenomenal paper is made. </p> <p>In request to concoct own style it's conceivable to use English paper tests that you may find on the web. So EssaysBank gives you an opportunity to buy amazing specially composed papers. Course for premium quality paper is just another subject presentation. Incredible gathering of paper composing guides and totally free examples. </p> <h2> The Importance of Free English Essay Samples </h2> <p>English article tests should be completely broke down and comprehended for t he young understudy before attempting to form his perfect work of art. You need to see how to make a compelling exposition as it is a run of the mill establishment for an understudy's evaluation. On the off chance that you've just moved on from school or college and are attempting to get an awesome line of work, you should get a powerful resume to dazzle your future business. Scholastic papers can't contain any signs of counterfeiting. </p> <h2>Free English Essay Samples Can Be Fun for Everyone </h2> <p>From begin to finish a copper repiping work takes only several days. Utilize the guide of genuine scholarly specialists and get the administration you have earned! Our team of scholars who give this type of help will be glad to gain an occupation winning resume for you. Mention to our specialists what kind of schoolwork help on the web you need to get. </p> <p>Finally, in any case, recall that great composing doesn't occur coincidentally. Some cons ider article composing a weight while some observe it like an opportunity to impart their thoughts and insights. On the off chance that you are looking for help with your exposition, at that point we give an exhaustive composing administration given by completely qualified scholastics in your general vicinity of study. Composing a paper is taking out a decent arrangement of your vitality directly from endeavoring to build up a theme. </p>
Monday, May 11, 2020
What is a Citation in a Research Paper?
<h1>What is a Citation in a Research Paper?</h1><p>What is a reference? In a paper, the understudy or scientist composes a solitary reference to an outside source to delineate the criticalness of what is being presented.</p><p></p><p>Citations can be incorporated toward the finish of the paper or can be composed independently. They ought to be elegantly composed and very much reported, and ought to be sourced to essential sources just (when possible).</p><p></p><p>Authorship: When composing references, the essayist ought to demonstrate the name of the writer where conceivable. This is typically done by setting a caption after the creator's name, e.g., 'Creators' names toward the start of the text'Author toward the finish of the content'. The essayist ought not utilize condensed names (e.g., Lance) to show initiation; such shortened forms are once in a while utilized in distributed articles.</p><p></p> <p>Sources: If composing a reference, the author should source the reference effectively, utilizing the full and right reference wordings (e.g., the writer 'E. L. Mead, Department of Sociology, University of Minnesota'). As a kindness, the referencing data can be remembered for the bibliography.</p><p></p><p>Style: When composing references, they ought to be appropriately organized (e.g., commentary sources must be without spaces or other unique arranging), including source and asset data as fitting. Redundancy of source and asset data is satisfactory, yet the source and asset data ought to be steady all through the paper. Language and organizing rules are equivalent to those utilized in a creator's note.</p><p></p><p>Authors: Authorship: The creator's name ought to show up in the main sentence and ought to be trailed by the name of the creator (if essential) in every single resulting sentence. When composing references, the name s of all writers ought to be recorded in the book index (the references ought to be recorded in the request in which they were composed, for accommodation of reference and readers).</p><p></p><p>Author's Note: All references ought to incorporate the writer's name (i.e., 'B.A. by Robert W. Corridor'). Redundancy of the writer's name in a similar sentence is worthy, be that as it may, as on account of creation, this ought not be done as a major aspect of the refering to data (for appearance and familiarity).</p><p></p><p>Another update: journalists who compose references for an understudy or analyst ought to think of them from the point of view of the peruser, instead of from the perspective of the writer. At the end of the day, references ought to be about the essayist and what is being composed, not about the writer and what is being said.</p>
Know What an Old Pro Thinks About Journal Writing Services
<h1> Know What an Old Pro Thinks About Journal Writing Services</h1> <h2>Ok, I Think I Understand Journal Writing Services, Now Tell Me About Journal Writing Services! </h2> <p>After having a total handle of the investigation, after that you can make a generous and fitting survey. The administrations gave by us are additionally very supportive for local speakers of English who'd incline toward another pair of expertly prepared eyes to be certain that the work they submit for diary distribution comprises of no blunders or irregularities and is generally cleaned to flawlessness. Each time you present an article some place for people to peruse, verify that it fulfills the best guidelines conceivable. You don't simply remark about a particular article without completely grasping its substance. </p> <h2> New Ideas Into Journal Writing Services Never Before Revealed </h2> <p>Cultural Differences If you're trusting your piece will be per used by an enormous number of people, it is justified, despite all the trouble to consider that various them will have broadly various convictions, practices and practices rather than you. In the event that you get to a level any place your negative musings outperform positive contemplations, your emotional wellness will be disintegrated. There may be individuals' activities which you find disagreeable, methods for doing things which aren't as you would do them, workplaces in which you wouldn't wish to remain. For some of them you will need support, while others you'll have the option to compose completely all alone. </p> <p>Make any changes in accordance with the thinking of you feel need to get made. Less frequently you're ready to utilize your diary to create decisions about something in your locale administration environment. There are a few inventive and gainful techniques diary composing may be used in treatment. Despite the fact that diary composing might be utili zed in boundless manners, there are a couple of general procedures that most advisors use. </p> <h2> The Hidden Secret of Journal Writing Services </h2> <p>When you employ us, you can be certain you're ready to get the paper which you truly need. Diary templates are typically quiet with respect to the interest for digests to be carefully composed, meaningful and useful. At that point you can pick on the off chance that you might want to keep journaling. Make certain to record your paper successfully!</p> <h2>A Secret Weapon for Journal Writing Services </h2> <p>The stage welcomes understudies to fill in a shape and after that pick the most appropriate essayist. Our skilled scholars are here so as to help you in explaining any scholastic undertaking. In addition, you must be brief in all segments of the article.</p> <p>If you request from us, you can discover a draft of your paper to make certain your author is going the best possible way. Sharpen Your Writing Skills If you're seeking to be an author, diary composing can work as the absolute best exercise. Journal authors frequently wonder how to begin. Picking on the web article essayists isn't a bad dream any longer. </p> <h2> Journal Writing Services - Overview </h2> <p>What you need to do is to round out the buy structure, make an installment, and in a few minutes, you will get your individual article aide. You need to secure entire perception of the data you will use to make your logical audit. Join a club with respect to a subject that you wish to discover increasingly about. You're ready to just telephone, email or utilize our site to start the discussion. </p> <p>Moreover, our webpage blog comprises of free composing tips and instructional exercises to help planned customers notwithstanding for u nderstudies who just require scholastic direction. In this manner, to form a Meta examination, you ought to be an expert recorded as a hard copy notwithstanding in insights to dissect the outcomes precisely. While a casual and now and again ungraded task, diary sections can assist you with creating other transferable composing capacities. At that point your aide will start to make a task for you. </p> <p>There are a lot of critical points of interest to recording things, however they're eccentric and hard to reliably catch without a decent propensity set up. There are tons and bunches of points of interest to keeping a diary and various them have been logically demonstrated. There are heaps of things that we should dobut we don't do them. Maybe you could start your day by making a rundown of a portion of your inclinations, things you need to do, ventures that need to be done, courses you need to take, places you couldn't want anything more than to visit and books you nee d to peruse, and so forth </p> <p>Every report made by our essayist have a top notch ensure or your cash back. Our authors have colossal comprehension of getting to different insightful material online from other database. Prepared authors make unique materials for each and every client. Just fill in the buy structure on the webpage, counsel our client assistance if necessary, and extremely soon the absolute most noteworthy diary article journalists to be found on the Internet will do their absolute best to set up your optimal task for you. </p> <p>You want a business with a strong rockreputation. The business ensures secrecy and worthy discount if necessary. It utilizes master authors having some expertise in different fields. It utilizes an individual way to deal with each customer and it positively affects their scholastic accomplishments. </p> <p>Our rates are moderate for some understudies. You can even look at a few surveys and pick the business that is generally reasonable for your prerequisites and truly moderate financial plan. The survey is going to show you anything you desire to comprehend and afterward it is conceivable to put your buy certainly. Our surveys contain data like the score of the customers, our rating, beginning costs, Discounts, and grade of the papers. </p>
Friday, May 8, 2020
Facts, Fiction and Research Paper Topics Ideas about Paralysis
<h1> Facts, Fiction and Research Paper Topics Ideas about Paralysis </h1> <p>The supreme most significant issue looked by understudy is the deficiency of insight and expertise to examine on a particular subject. In case you're expounding on social issues, you will generally be expounding on the manner in which the point influences bigger gatherings of individuals, similar to how neediness influences an entire age. Once in a while dubious issues can be interesting to investigate. Inquiring about any issue about government or laws may get overpowering in light of the multifaceted nature of the issues and even by virtue of the wording of some laws.</p> <p>Research composing will keep being a piece of their ordinary learning educational program, and it's something they should succeed. Understudies frequently require direction in exploring. </p> <p>Students can't build up a generally excellent object of partiality articles, not on the grounds that they can't compose papers yet likely because of the straightforward certainty which they can't make prevalent titles for papers on preference. In an all around created section, they ought to have the option to underline the sentence that is the POINT. Thus, they can think of various proposal for various topics to compose articles on village. The understudies will have a great time making their exploration beside fearing it. </p> <h2>Research Paper Topics Ideas about Paralysis - Is it a Scam? </h2> <p>Pick the subject you'd prefer to look into about in your work. Whenever you're watching out for an examination theme for your investigations and couldn't imagine anything better than to focus on wellbeing brain science, consider the recommendations that follow. A mind boggling research paper subject won't simply take parcel of time yet also probably won't be effectively intelligible because of its multifaceted nature. There are a few distinct subjects which similarly have a phenomenal scope of research under the anomalous brain science umbrella. </p> <p>Below you'll locate a few potential proposals for inquire about papers on dreams. Fun research subjects may even transform into a methods for them to find that composing is an ability they are in truth awesome at. You've looked to a great extent to get familiar with a perfect point for inquire about however the entirety of your endeavors have gone futile. </p> <h2> Top Choices of Research Paper Topics Ideas about Paralysis</h2> <p>Conclusion The choice of the tweaked look into papers have become the most important single part of it. The individual evaluating your paper will in all probability acknowledge how you strayed from the standard. Hold on to compose that when it is finished and you can perceive what you are presenting. You might want your paper to be seen, which means you don't wish to pick the easy theme and have your paper reflect the very same thing each one's paper does. </p> <h2>The Dirty Truth on Research Paper Topics Ideas a bout Paralysis </h2> <p>You would be approached to introduce contentions for your examination in a reasonable and succinct manner. Preferably fundamental foundation data should be accessible however data on the specific research paper should be restricted. Your determination of a legitimate research subject is frequently controlled by whether the case you're keen on perusing or exploring. Consider various configurations or sorts of papers there are, and the different kinds that you've composed. </p> <p>Evidently, you won't can make an examination paper if adequate data concerning the subject isn't advertised. In case you're mistaken for various intriguing points to inquire about on the web, it's far superior to figure out what intrigues you the most. You'll likewise realize what data to attempt to discover straightaway. Now and again the data capacities as a springboard to discovering more top to bottom data elsewhere.</p> <h2> The Benefits of Research Paper Topics Ideas about Paralysis </h2> <p>Proceed to the web gatherings, there can be one your theme, and see what's being examined. Prior to starting, ensure that the point you're keen on is with regards to the specific task. Actually, you probably won't have been expanded a specific theme in any capacity. It very well may be trying to think about a theme off the pinnacle of your head. </p> <p>You ought to be very cautious in the assortment of the theme. The theme should be adequate to help you reach to a particular resolution. It ought to be wide with a decent extension and it ought to have the option to produce a great deal of contentions and thoughts for you. Contentious paper subjects are so significant since they are debatableand it's basic to consistently be fundamentally thinking about our general surroundings. </p> <p>Basically in any case, the thought is to avoid making presumptions that not many or no adequate people would make. It's energetically suggested that you simply pick the subject that it is conceivable to manage, for example, on the off chance that you're not t portraying the character attributes, at that point you have to better not choose for it. While choosing a definition, recall that there are numerous sorts of moral contentions and that the heading you contend for your particular case depends in enormous part on how you charac terize your terms. The absolute first bit of making this sort of contention is regularly setting up that we tend to think about and feel for various people. </p> <h2> The Basic Facts of Research Paper Topics Ideas about Paralysis</h2> <p>In that circumstance, you might need to expect the nearness of God in your initial section instead of spending a few pages giving a contention to the nearness of God. Realities, at last, will consistently win out against how people are feeling at a particular second. You won't just be in a situation to advance your thoughts in most ideal design be that as it may, you will likewise have a great time composing it. A fantastic way to deliver novel and exceptional thought is to investigate web or figure the most sizzling theme in the news. </p>
Writing Persuasive Essay Topics on Environment
Writing Persuasive Essay Topics on EnvironmentOne of the best reasons for writing persuasive essays is to demonstrate the harm that our oil and gas industry are doing to our planet. There are many reasons for this, but one of the most prominent is the harm that our gas and oil industry are doing to our land and water. The fact that our oil and gas industry are so great in part because it can use a lot of natural resources which are essential to our lives as a nation, but at the same time they use very little of those resources which might be considered waste or spoilage from those natural resources.The problem with using this type of argument to persuade a person to change their stance on certain issues of importance such as the issue of global warming, or the ozone layer, is that humans use all of those natural resources that our government doesn't necessarily regulate themselves. If the government did regulate our oil and gas industry then it would be difficult to use all those nat ural resources without causing problems. Therefore to use this argument it is necessary to show people that we have a problem.The best way to apply this persuasive essay topics on environment to persuading people to change their viewpoint is to make the point that humans can not continue to use natural resources without causing problems for the natural environment. Humans can use natural resources that are not renewable; they can use polluting fossil fuels in large quantities; they can release harmful pollutants into the air and soil; and they can destroy the delicate balance of the earth. We have become too dependent on these resources and without them, we cannot exist in today's world.Another persuasive essay topics on environment to successfully persuade people to do something about environmental issues is to create an image of the environmentalist as someone who is interested in helping others. By creating an image of the environmentalist as someone who is concerned about others and who is willing to take action in order to help others, we create an image of someone who is caring and compassionate and someone who will do whatever they can to help make a better world. This is a great way to persuade people to do something.Because we are currently using a natural resource that is depleting rapidly, we have the opportunity now to save ourselves from the problems we have created. The important thing to remember is that we have the ability to go back to the time when we were 'natural', before the use of our natural resources was rampant. We can get back to using natural resources that we are naturally suited for and to do so we must do something now.Presenting persuasive essay topics on the environment in a positive light in order to convince people to change their stance is the best way to go about changing minds and altering the opinion of those people who might already be thinking against change. Having an answer to a problem, a solution, is the first step i n getting what you want from the situation. If you can provide an answer to the question, 'What can I do to help make the world a better place,' then you have an answer to your question.The argument, such as the one you are presenting to the reader, is always effective, but to convince someone to do something can be more difficult. Persuasion is a process of creating an image of someone in their mind that helps create a connection between their own personal beliefs and reality. When you can do this, then you have effectively changed someone's opinion and you have made them do something about their belief.Writing persuasive essay topics on environment and convincing others to make the changes you wish to see in our world requires an understanding of reality and a desire to do something about it. Making the case for doing something about the environmental problems we have created is a logical way to get people to see the world through your eyes.
Wednesday, May 6, 2020
Questions On Equity And Education - 1741 Words
Equity There are many problems that equity is facing that need to be corrected. It has been a focus to improve the issues that equity acquires, but the efforts have failed since it has been a problem for decades. It can change the way students are learning and it will allow everyone to have the resources needed. Equity is of great importance in education, but it has many issues that go with it. The issues affect many children and families in the community. Finding a solution to THREE-YEAR PLAN AND 5 ISSUES IN EDUCATION 5 the problem is necessary and there are some solutions in place that hopefully will help.â⬠¦show more contentâ⬠¦There has also been a problem with tracking students and the way that the school sorts them out. Depending on race, low academics, special ed. and the non-white students are also disproportionally represented and subject to discipline policy that removes them from school (Nielson, 2013). Poverty Poverty is a huge issue in a childââ¬â¢s educational success. That does not mean that children living in poverty will never be successful, but the chances of that are slimmer. It can affect the way children react in academics and in social situations too. Most of the time the effects of child poverty cannot be prevented, but one can facilitate these children for a better future. It can be programs, educators, family, or anyone willing to help a child in need. Children cannot achieve the goals that they need in school when they are in poverty and have no help. Not only because they are poor, but because of the stress that poverty creates for these children. Stress is a big topic that affects a childââ¬â¢s life as they grow up to become adults. It is not just stress itself, but THREE-YEAR PLAN AND 5 ISSUES IN EDUCATION 6 how the body reacts to it that affects the brain. ââ¬Å"As a result, children who grow up in stressful environments generally find it harder to
Fish Phylogenetic Tree Based on Protein Size free essay sample
Fish phylogenetic tree based on protein size Amanda Reed Lab partners: Lecia Redwine, Kyle Hatcher TA: Baneshwar Singh Biology 117, Section 25 Tuesday 10:30 AM Introduction Although tree diagrams have been used since the days of Charles Darwin, biologists have only recently adopted the tree model of evolution to read and interpret phylogenies. One of the reasons for this is the confusion that often arises from using a tree model to describe a phylogeny (Baum, 2008a). Many people interpret the trees to show that different species evolve from one another instead of viewing them as ways to trace the common ancestors between species. All species at the tips of the branches should be thought of as being evolutionarily equal; however, many people misinterpret the trees to show how different organisms evolved from one another (Baum, 2008b). Phylogenetic trees are a great way to represent how evolution led to the differentiation of species. However, to determine how to draw the tree, one must first define what a species is. Unfortunately, there is no black-and-white answer to determining the existence of new species. First, though, one must decide if a new organism is different enough from pre-existing species to constitute a new species or not. One must also have a method to detect new species. According to Ernst Mayr, organisms of the same species are able to reproduce fertile offspring. However, George G. Simpson argued that members of the same species have the same evolutionary history. Today, the two ideas have been combined to create the Biological Species Concept. This is still open for individual interpretation, so scientists, for the most part, have chosen to follow the Phylogenetic Species Concept which uses the most accurate phylogenetic trees available depicting organisms with shared traits to determine if an organism is a new species or not. Currently, the easiest way to depict the most accurate phylogenetic tree without using computer software is to attempt to determine the most parsimonious tree. If organisms have many shared traits, then they can be determined to be of the same species as each other (Hey, 2009). Another key piece of information used in determining phylogenetic trees is the evolutionary rate. Biologists often use the evolutionary rate to calibrate a ââ¬Å"molecular clockâ⬠à to determine an evolutionary timeline for a species for which we may not have much evolutionary information. This helps to determine missing pieces in a phylogenetic tree, thus allowing us to create a phylogenetic tree when we may not have all of the information. We can presume that the tips of the tree are equally evolved, as well (Ho, 2008). By using a relaxed molecular clock that presumes that the evolutionary rate varies between organisms along with the Phylogenetic Species Concept, one can get an idea of a phylogenetic tree for a set of organisms, based on their traits. The purpose of this lab was to try to determine a phylogenetic tree for six different fish using the proteins each fish contained to determine their placement on the tree. Methods 3 flip top microtubules and 3 screw top microtubules were labeled. 250 microliters of Laemmli sample was added to each flip top microtubule. A small piece of each fish sample was added to its designated flip top microtubule. Each flip top microtubule was then agitated by flicking it approximately 15 times with a fingertip. The samples were then incubated at room temperature to separate and extract the fish proteins. The buffer solutions containing the fish proteins were then transferred into their designated screw top microtubules. These microtubules were then heated for 5 minutes at 95 degrees Celsius to denature the proteins. The samples were then stored at temperatures less than -20 degrees Celsius until the following lab meeting. The next lab meeting, the frozen fish samples and actin and myosin samples were reheated at 95 degrees Celsius to redissolve any precipitated detergent. The gel box was assembled, and the polyacrylamide gel was inserted into the vertical electrophoresis unit. TGS buffer was then added to the electrophoresis unit until it was above the top of the smaller plate on both the inside and the outside of the chamber. The wells were then loaded with the protein samples using a micropipetter with thin gel loading tips. The first well remained empty. The second and sixth wells were loaded with Precision Plus Protein Kaleidoscope prestained standard. The third well was loaded with mahi-mahi protein sample. Well four was loaded with salmon protein sample. The fifth well was loaded with catfish protein sample. Well seven was loaded with sardine protein sample. Well eight was loaded with flounder protein sample. The ninth well was loaded with shark protein sample. Well ten was loaded with actin and myosin standard proteins. After all samples were loaded, the lid was placed on the tank and the leads were inserted into the power supply. The voltage was set to 200 V, and the gel ran for 30 minutes. After the gel was ran, the power supply was disconnected and the lid removed. The buffer was poured out of the electrode assembly, and the gel was removed. The gel plates were pried apart. Then the gel was rinsed three times with water for five minutes to improve the intensity of the protein bands. The water was removed, and 50 milliliters of Bio-Safe Coomassie stain was added. The gels were stained for a minimum of an hour. The stain was then removed and replaced with a large volume of water overnight. Once the gel had been destained and dried, the proteins were then scored. This was done by measuring the distance from the wells to the known bands on the Precision Plus Protein Kaleidoscope and graphing the distances versus the weight in kilodaltons on logarithmic graph paper. A best fit line was drawn using these points. Then the distances were measured from the wells to the bands of the proteins from the fish samples. Using the best fit line drawn on the logarithmic graph paper, the band lengths of the fish proteins were translated to protein weights in kilodaltons. These weights were then used to create a matrix. This matrix was then entered into MacClade to make a phylogenetic tree. The branches of the tree were then moved around so that the most parsimonious tree could be created. The most parsimonious tree, in this case, was determined by the tree with the shortest length. Results The polyacrylamide gel showed bands of proteins based upon their weight. The further away from the gel the protein band occurred, the heavier the protein was. The Precision Plus Protein Kaleidoscope prestained standard and actin and myosin standard ladders were very neatly spaced out, and one could easily detect the presence of different proteins. However, the fish proteins were not so easily discerned, especially since, in most cases, the lighter proteins had less color. Wells 2 and 6 contained Precision Plus Protein Kaleidoscope prestained standard. This formed a neat, ladder-like set of bands which were easily used to determine the weights of the other proteins. Based upon the weight versus the distance from the wells, we found 26 different proteins among the six fish. Both sharks and sardines appear to share few proteins with the other fish, while salmon, catfish, mahi-mahi, and flounder appear to be more closely related, and they share at least a few proteins between themselves. No one protein appeared in all fish. Figures and Tables This is a picture of the gel that we ran. From left to right, the wells were numbered 1 through 10. Well 1 remained empty. Well 2 had 5 microliters of Precision Plus Protein Kaleidoscope prestained standard. Well 3 contained 10 microliters of mahi-mahi protein sample. Well 4 contained 10 microliters of salmon protein sample. Well 5 contained 10 microliters of catfish protein sample. Well 6 contained 5 microliters of Precision Plus Protein Kaleidoscope prestained standard. Well 7 contained 10 microliters of sardine protein sample. Well 8 contained 10 microliters of flounder protein sample. Well 9 contained 10 microliters of shark protein sample. Well 10 contained 10 microliters of actin and myosin standard. This is the matrix created using MacClade. The right column gives the names of each fish sample. The top row gives the weight of each protein in kilodaltons. In the picture of the phylogenetic tree that follows, the protein weights are represented by 1 through 26, with 1 being the heaviest and 26 being the lightest. Where the numbers are indicates that protein was either lost or gained at that point. The tree that was generated had a tree length of 31. According to MacClade, the minimum length was 26; however, we were not able to find a tree with a length shorter than this. Discussion No one protein was shared among all six fish. Sharks and sardines appeared to both be outgroups, based upon our results. The shark could easily be an outgroup since it has more cartilage instead of dense bone. The sardines are less different physically from the other fish, so it is unclear why this fish would also be an outgroup. The tree that was created using MacClade had a length of 31. The minimum length was 26, but we were unable to get any closer to the minimum than 31. The results may have been somewhat skewed because the same proteins may have been measured to be slightly different lengths, causing more proteins to have been found than really existed. More fish may have shared more proteins than what we found.
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